Caspase-1 Assay Kit (Fluorometric) from Abcam

Product Specs
ItemCaspase-1 Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab39412
Quantity100 test
Sample TypeTissue Extracts, Cell Lysate
Time2h 0m
ApplicationsCellular Assay
Gene NameCASP1
TypeEnzyme activity
NCBI Gene AliasesICE, IL1BC, P45
TargetCaspase-1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Polyphenol-rich diet mediates interplay between macrophage-neutrophil and gut microbiota to alleviate intestinal inflammationCell Death & DiseaseOctober 9, 2023Dandan Han, Yujun Wu, Dongdong Lu, Jiaman Pang, Jie Hu, Xiangyu Zhang, Zhenyu Wang, Guolong Zhang, Junjun WangCaspase-1 and NLRP3 activation in colonic and cellular lysis was measured using Caspase-1 Assay Kit (ab39412, Abcam, Cambridge, MA, USA) and NLRP3 ELISA Kit (ab279417, Abcam, Cambridge, MA, USA), respectively,

(2) Enhanced Activity of NLRP3 Inflammasome in the Lung of Patients with Anti-Synthetase SyndromeCellsDecember 23, 2022Espiridión Ramos-Martinez, Angel E. Vega-Sánchez, Gloria Pérez-Rubio, Mayra Mejia, Ivette Buendía-Roldán, Montserrat I. González-Pérez, Heidegger N. Mateos-Toledo, Warrison A. Andrade, Ramcés Falfán-Valencia, Jorge Rojas-SerranoAccording to the manufacturer’s instructions, the activity was detected using a Caspase-1 assay kit (ab39412, Abcam™, Cambridge, UK). All assays were performed in triplicate in three independent experiments. 2.7.

(3) Synthetic Secoisolariciresinol Diglucoside (LGM2605) Prevents Asbestos-Induced Inflammation and Genotoxic Cell Damage in Human Mesothelial CellsInternational Journal of Molecular SciencesSeptember 3, 2022Ralph A. Pietrofesa, Shampa Chatterjee, Yuwaraj Kadariya, Joseph R. Testa, Steven M. Albelda, Melpo Christofidou-Solomidouactivity was determined using a commercially available fluorometric assay kit (Caspase-1 Assay Kit, ab39412, Abcam, Waltham, MA, USA), as previously performed [15]. Briefly, the assay kit measures caspase-1 activity

(4) Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytesFrontiers in ChemistryAugust 10, 2022Xiao Wang et al.fluorescence microscope. For the caspase-1 activity assay, the Caspase-1 assay kit (Fluorometric) (#ab39412, Abcam) was used. In brief, cell lysates were prepared after treatment. Reaction buffer and YVAD-AFC

(5) Galacto-oligosaccharides alleviate lung inflammation by inhibiting NLRP3 inflammasome activation in vivo and in vitroJournal of advanced researchJuly 1, 2022Cai Y, Gilbert MS, Gerrits WJJ, Folkerts G, Braber S.assay Caspase-1 activity in PBECs and A549 cells was determined in 50 μL cell lysates using a commercial kit (ab39412, Abcam, Cambridge, UK) according to the manufacturer’s instructions. For the in vivo experiments, same

(6) MicroRNA-1224-5p Aggravates Sepsis-Related Acute Lung Injury in MiceOxidative Medicine and Cellular LongevityJune 28, 2022Bing Liu, Feng Chen, Ni-Tao Cheng, Zheng Tang, Xian-Guo Wang, Ming Xu(ab102526), myeloperoxidase (MPO) activity assay kit (ab105136), and caspase-1 activity assay kit (ab39412) were all purchased from Abcam (Cambridge, MA, USA). TransAM® NF-κB p65 kit (40097) was purchased from

(7) Role of Polyphenol-Derived Phenolic Acid in Mitigation of Inflammasome-Mediated Anxiety and DepressionBiomedicinesMay 28, 2022Ruth Iban-Arias, Maria Sebastian-Valverde, Henry Wu, Weiting Lyu, Qingli Wu, Jim Simon, Giulio Maria Pasinetticaspase-1 (Cat#ab39901, Abcam, Cambridge, UK), and the fluorometric Caspase-1 Assay Kit (Abcam, Cat#ab39412, Abcam, Cambridge, UK), by following the protocol established by the fabricator. 2.1.9. THP1 Macrophages THP1

(8) RGS6 Drives Spinal Cord Injury by Inhibiting AMPK Pathway in MiceDisease MarkersApril 25, 2022Wenxin Dao, Zhe Xiao, Weize Yang, Xiaomin Luo, Hongxia Xia, Zuneng Luand tumor necrosis factor-alpha (TNF-α, ab208348) ELISA kits, and fluorometric caspase-1 assay kit (ab39412) were purchased from Abcam (Cambridge, MA, USA). Lentiviral vectors carrying short hairpin RNA against

(9) TRPV1 Hyperfunction Contributes to Renal Inflammation in Oxalate NephropathyInternational Journal of Molecular SciencesJune 8, 2021Chien-Lin Lu, Te-Yi Teng, Min-Tser Liao, Ming-Chieh Main cell lysates and renal cortical tissues was determined using a commercial fluorometric assay kit (ab39412) obtained from Abcam (Bristol, UK) and expressed as units/mg of protein. 4.16. Statistics Numerical

(10) Deubiquitination of NLRP6 inflammasome by Cyld critically regulates intestinal inflammationNature ImmunologyJune 1, 2020Sandip Mukherjee, Ritesh Kumar, Elviche Tsakem Tsakem Lenou, Venkatesha Basrur, Dimitris L. Kontoyiannis, Fotis Ioakeimidis, George Mosialos, Arianne L. Theiss, Richard A. Flavell, K. Venuprasadactivity assay. The activity of caspase-1 was determined in colon homogenates by a commercial kit (Abcam, ab39412) according to the manufacturer’s instructions. Briefly, fresh frozen sections of colons were homogenized

(11) Haematopoietic stem cell gene therapy with IL ‐1Ra rescues cognitive loss in mucopolysaccharidosis IIIA EMBO Molecular MedicineMarch 6, 2020Helen Parker et al.1 activity was measured using 100 μg total brain protein with the Caspase 1 Fluorometric Assay Kit (ab39412) as per the manufacturer's protocol (Abcam, UK), using recombinant mouse caspase 1 (0.02‐2U; Abcam)

(12) Tojapride prevents CaSR‐mediated NLRP3 inflammasome activation in oesophageal epithelium irritated by acidic bile saltsJournal of Cellular and Molecular MedicineJanuary 1, 2020Xiao‐Lan Yin et al.Measurement of caspase‐1 activity The activity of caspase‐1 was detected using a Caspase 1 Assay kit (ab39412, Abcam) per the manufacturer's instructions. All assays were performed in triplicate in three independent

(13) The pore-forming subunit Kir6.1 of the K-ATP channel negatively regulates the NLRP3 inflammasome to control insulin resistance by interacting with NLRP3Experimental & molecular medicineAugust 6, 2019Du RH, Lu M, Wang C, Ding JH, Wu G, Hu G.pcDNA3-N-Flag-NLRP3 (75127) was obtained from Addgene (Watertown, MA, USA). A caspase-1 activity assay kit (ab39412) was obtained from Abcam (Cambridge, UK). All other materials were obtained as described elsewhere22. Mice Kir6.1

(14) Immunoglobulin light chains generate proinflammatory and profibrotic kidney injuryThe Journal of clinical investigationJune 17, 2019Ying WZ, Li X, Rangarajan S, Feng W, Curtis LM, Sanders PW.Millipore Sigma); a Caspase-1 Colorimetric Assay Kit (Ab39470, Abcam) or a Caspase-1 Fluorometric Assay Kit (Ab39412, Abcam); a Mouse TGF-β1 ELISA Kit (catalog LS-F5184-1, LifeSpan BioSciences); a Mouse Albumin ELISA Kit

(15) Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP PathwayJournal of diabetes researchJanuary 1, 2019Wei H, Bu R, Yang Q, Jia J, Li T, Wang Q, Chen Y.Activity Assay. Caspase-1 activity was determined using the Caspase-1 Assay Kit (ﬂuorometric) from Abcam (ab39412, UK) according to the manufacturer’s instructions. Brieﬂy, cells were lysed and the nuclei and organelles

(16) Involvement of inflammasome activation via elevation of uric acid level in nociception in a mouse model of muscle painMolecular painJanuary 1, 2019Yoshida S, Hagiwara Y, Tsuchiya M, Shinoda M, Koide M, Hatakeyama H, Chaweewannakorn C, Suzuki K, Yano T, Sogi Y, et al.Uric Acid Colorimetric/Fluorometric Assay Kit (K608-100, BioVision Inc., Milpitas, CA, USA) and Caspase 1 Assay Kit (ab39412, Abcam plc), respectively. Tissue samples were disrupted and homogenized using buffers from each kit.

(17) CaMKIIδ-mediated inflammatory gene expression and inflammasome activation in cardiomyocytes initiate inflammation and induce fibrosisJCI InsightJune 21, 2018Andrew Willeford, Takeshi Suetomi, Audrey Nickle, Hal M. Hoffman, Shigeki Miyamoto, Joan Heller BrownNRVMs using a prevalidated Fluorometric Caspase-1 Activity Kit as described by the manufacturer (Abcam, ab39412). Immunoblotting. Western blot analysis was performed on snap-frozen ventricular tissue. Tissue samples were

(18) Carnosic acid prevents dextran sulfate sodium-induced acute colitis associated with the regulation of the Keap1/Nrf2 pathwayScientific ReportsDecember 1, 2017Neng Yang, Zongling Xia, Naiyuan Shao, Bowen Li, Lian Xue, Ya Peng, Feng Zhi, Yilin Yangcentrifugation at 10,000 g for 10 min. The activity of caspase-1 was determined by a commercial kit (Abcam, ab39412) according to the manufacturer’s instructions. Immunofluorescence histochemistry For immunofluorescence

(19) Developmental Role of Macrophage Cannabinoid-1 Receptor Signaling in Type 2 DiabetesDiabetesApril 1, 2017Tony Jourdan et al.Inﬂammation Caspase-1 activity was determined using the Caspase-1 Assay Kit (Fluorometric) from Abcam (ab39412); interleukin (IL)-1b and tumor necrosis factor-a (TNF-a) proteins were measured using the Rat Quantikine

(20) PKM2-dependent glycolysis promotes NLRP3 and AIM2 inflammasome activationNature communicationsOctober 25, 2016Xie M, Yu Y, Kang R, Zhu S, Yang L, Zeng L, Sun X, Yang M, Billiar TR, Wang H, et al.

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Caspase-1 Assay Kit (Fluorometric) from Abcam

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Caspase-1 Assay Kit (Fluorometric) from Abcam

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